Zeyuan Wang

Zeyuan Wang

I am studying the kinetics of reversible enzyme inhibition using diazepam as substrate for human CYP3A4/5. The goal is to confirm reversible inhibitory kinetics of CYP3A4/5 for this drug using enzyme incubation assays and LC-MS methods.  Based on the numerical method in Mathematica software, I can better understand the primary parameters (e.g., Km, Vmax, kcat) of enzyme kinetics.