Proteomics is widely and successfully used in drug discovery, disease treatment research, and biomarker discovery. These intricate studies require high levels of quality controls, adequate instrumentation, and expertise to analyze thousands of proteins to obtain meaningful results.
The leadership of the Temple Proteomics Facility embraces these principles and invests continuously by recruiting experts, procuring hi-tech equipment, and regularly revising protocols and procedures. The staff are experts in proteomics, tissue microanalysis, and relevant bioinformatics platforms. Research at the Facility has led to numerous scientific discoveries and has been extensively published and cited.
The Facility assists researchers with state-of-the-art mass spectrometry expertise, practice, and instrumentation to address health-related scientific questions concerning our community. At the Facility, researchers can study the proteome, including modifications made to a particular set of proteins produced by a cell, tissue, organism, or system.
The Facility is located at the Temple University School of Pharmacy on the Temple University Health Sciences Center Campus. The facility was established in 2005 as part of the Temple University Researcher Development Program and provides accessible proteomics capability for biological and biomedical research to support faculty within the University and outside institutions, including pharmaceutical companies.
Examples of Services
- Protein expression profiling
- Protein identification
- Post-translational modification characterization
- Isolation of individual cells from tissues
- Protein and peptide separations
- Generation of high fidelity data for biomarker discovery, drug targets, identification of molecular transducers of disease
- Performance of multiple protein assays, including modified iST, GeLCMs, SILAC, post-translational modifications, MRM/SRM, iTRAQ, DARTS, protein-protein interactions
TSQ Quantum Ultra™ Triple Quadrupole Mass Spectrometer
Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer
Orbitrap Elite™ Hybrid Ion Trap-Orbitrap Mass Spectrometer with ETD
Autoflex II TOF/TOF System
WES ProteinSimple System
Beckman Coulter P/ACE MDQ capillary electrophoresis "nanotechnology" system with dual wavelength laser-induced fluorescence (LIF) detection system
Waters HPLC System (2795)
Leica VT1200 S - Vibrating Blade Microtome
Veritas Microdissection Instrument with LCM, Laser Cutting and Fluorescence Detection
Salim Merali, PD
Director and Professor
Mario C. Rico, MD
Research Assistant Professor
Carmen Merali, BS
Research Scientist/Lab Manager